Live cell immunogold labelling of RNA polymerase II
نویسندگان
چکیده
Labeling nuclear proteins with electron dense probes in living cells has been a major challenge due to their inability to penetrate into nuclei. We developed a lipid-based approach for delivering antibodies coupled to 0.8 nm ultrasmall gold particles into the nucleus to label RNA polymerase II. Focussed Ion Beam slicing coupled to Scanning Electron Microscopy (FIB/SEM) enabled visualization of entire cells with probe localization accuracy in the 10 nm range.
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عنوان ژورنال:
دوره 5 شماره
صفحات -
تاریخ انتشار 2015